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gdnf gfra1 mixed  (R&D Systems)


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    R&D Systems gdnf gfra1 mixed
    Figure 1. RET activation with <t>GDNF/GFRa1</t> has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)
    Gdnf Gfra1 Mixed, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdnf+gfra1+mixed/Recombinant+Human+GDNF+Protein/pm37921725-63-7-14
    Average 96 stars, based on 458 article reviews
    gdnf gfra1 mixed - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs)."

    Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).

    Journal: Cytotherapy

    doi: 10.1016/j.jcyt.2023.10.003

    Figure 1. RET activation with GDNF/GFRa1 has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)
    Figure Legend Snippet: Figure 1. RET activation with GDNF/GFRa1 has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)

    Techniques Used: Activation Assay, Derivative Assay, Microscopy, Staining, Colony-forming Unit Assay

    Related Articles

    Activation Assay:

    Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).
    Article Snippet: The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.. For the GDNF/GFRa1 experiment, 100 ng/mL of GDNF & GFRa1 mixed 1:1; (212-GD-010, 714-GR-100; R&D Systems, France) were added to the media.. Cells were thereafter washed and resuspended in PBS.Cells were thereafter washed and resuspended in PBS.

    Derivative Assay:

    Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).
    Article Snippet: The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.. For the GDNF/GFRa1 experiment, 100 ng/mL of GDNF & GFRa1 mixed 1:1; (212-GD-010, 714-GR-100; R&D Systems, France) were added to the media.. Cells were thereafter washed and resuspended in PBS.Cells were thereafter washed and resuspended in PBS.

    Microscopy:

    Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).
    Article Snippet: The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.. For the GDNF/GFRa1 experiment, 100 ng/mL of GDNF & GFRa1 mixed 1:1; (212-GD-010, 714-GR-100; R&D Systems, France) were added to the media.. Cells were thereafter washed and resuspended in PBS.Cells were thereafter washed and resuspended in PBS.

    Staining:

    Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).
    Article Snippet: The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.. For the GDNF/GFRa1 experiment, 100 ng/mL of GDNF & GFRa1 mixed 1:1; (212-GD-010, 714-GR-100; R&D Systems, France) were added to the media.. Cells were thereafter washed and resuspended in PBS.Cells were thereafter washed and resuspended in PBS.

    Colony-forming Unit Assay:

    Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).
    Article Snippet: The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.The medium was changed according to the manufacturer’s instruction and floating cells were harvested on day +13 of hematopoietic differentiation.. For the GDNF/GFRa1 experiment, 100 ng/mL of GDNF & GFRa1 mixed 1:1; (212-GD-010, 714-GR-100; R&D Systems, France) were added to the media.. Cells were thereafter washed and resuspended in PBS.Cells were thereafter washed and resuspended in PBS.



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    gdnf gfra1 mixed  (R&D Systems)
    96
    R&D Systems gdnf gfra1 mixed
    Figure 1. RET activation with <t>GDNF/GFRa1</t> has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)
    Gdnf Gfra1 Mixed, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdnf+gfra1+mixed/Recombinant+Human+GDNF+Protein/pm37921725-63-7-14
    Average 96 stars, based on 1 article reviews
    gdnf gfra1 mixed - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 1. RET activation with GDNF/GFRa1 has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)

    Journal: Cytotherapy

    Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).

    doi: 10.1016/j.jcyt.2023.10.003

    Figure Lengend Snippet: Figure 1. RET activation with GDNF/GFRa1 has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)

    Article Snippet: For the GDNF/GFRa1 experiment, 100 ng/mL of GDNF & GFRa1 mixed 1:1; (212-GD-010, 714-GR-100; R&D Systems, France) were added to the media.

    Techniques: Activation Assay, Derivative Assay, Microscopy, Staining, Colony-forming Unit Assay